By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such issues as environmental overview of biotechnological tactics and microbial changes of haloaromatic and haloaliphatic compounds.
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Meant for researchers in utilized microbiology and environmental engineers, this publication covers such issues as environmental review of biotechnological techniques and microbial variations of haloaromatic and haloaliphatic compounds.
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Although his method is not exactly comparable to the usual concept of drying from the liquid state it is also not freezing and drying from the frozen state. If one compares his results with those of other workers who lyophilized cultures in other suspending menstrua one is impressed by the high percentage survival of certain organisms. 3%of dried Brucella melitensis to be viable. In comparison, Heckly et al. 0% survived about 6 months’ storage at 2OOC. , storage temperature may be very critical.
RATEAND METHOD OF FREEZING Disagreement between authors as to the superiority of rapid freezing over slow freezing is probably a result of the differences in organisms, and/or suspending menstrua. If one believes, as do Proom and Hemmons ( 1949), Luyet ( 1951), and others, that cells are killed by penetration of the cell wall by ice crystals, then few if any cells would be killed by ultrarapid freezing. It is well established (Luyet, 1960a, b ) that the faster the rate of cooling the smaller the size of ice crystals formed and at sufficiently high freezing rates (several hundred degrees per second) there are no crystals formed, Methods of achieving rapid freezing, and the problems of controlling and measuring the rate of cooling, have been 32 ROBERT J.
B. NUTRITION AND AGE Aeration and nutrition during growth are as much a function or part of physiological cell age as is chronological age. Too frequently the age of the culture has been established without considering other factors that are involved. In fact, relatively few studies on lyophilization of bacteria have considered, as a variable, the conditions under which the organisms were produced. , implying, at least, that they considered these factors to have some influence on survival. Although a marked difference between cells grown on agar surface and submerged liquid culture might be expected this factor has scarcely been studied.